A Simple, Inexpensive, and Portable Image-Based Technique for Nondestructive Leaf Area Measurements

Cropped photo of a leaf in the ImageJ program prior to processing.

This new 6-page article, part of a series introducing various image-based measurements for horticultural research, introduces a simple, inexpensive, and portable image-based technique for nondestructive leaf area measurements. It uses an imaging apparatus made with ordinary office supplies to obtain leaf images in greenhouse or field environments. Leaf images are then processed and analyzed to measure leaf area using ImageJ, an open-source image processing program. Because both image capture and analysis are performed nondestructively, leaf area can be measured on the same leaf repeatedly, enabling the monitoring of leaf growth over time, as well as photosynthesis and transpiration. This technique is particularly useful to researchers and students studying leaf growth and physiology in greenhouse or field environments. Written by Shinsuke Agehara, Lillian Pride, Mariel Gallardo, and Jose Hernandez-Monterroza, and published by the UF/IFAS Horticultural Sciences Department.

LED light increases leaf area and root length of Humulus lupulus (var. Tettnanger) in vitro

Hops cones on the vine. Credit: Tyler Jones, UF/IFAS

Propagation of hops has traditionally been accomplished through vegetative techniques. A more modern technique, micropropagation, can be used to propagate hops and offers several advantages to vegetative techniques. Research examining the use of LED lights in plant production has observed its ability to promote growth in plants through emission of higher light quality, such as enhanced red and blue wavelength discharge. This new 5-page EDIS publication provides information on the use and application of LED lights to enhance leaf area and root length of hops. Written by Chi D. Nguyen, Dominic Vu, Heqiang Huo, and Brian Pearson, and published by the UF/IFAS Environmental Horticulture Department.