Isolation of Mother Cultures and Preparation of Spawn for Oyster Mushroom Cultivation

This 6-page publication details the cultivation of oyster mushrooms from mother culture isolation to spawn preparation. This protocol can be used by both homeowners and commercial cultivators. Written by Chih-Ming Hsu, Khalid Hameed, Van T. Cotter, and Hui-Ling Liao and published by the UF/IFAS Department of Soil and Water Sciences, January 2018.